Studies of multiple antioxidants against oxidative dam- age have both practical and theoretical significance due to two primary reasons. Balanced human diets contain multiple antioxidants. In addition to the antioxidative protection contributed by individual dietary antioxi- dants, strong evidence indicates that additive and syn- ergistic interactions occur among antioxidants. This synergism significantly strengthens the protection pro- vided by individual antioxidants alone.
Recently a heme protein spectra analysis program (HPSAP) has been used to evaluate the protection of multiple antioxidants against heme protein oxidation (OHP) in a tissue slice system or homogenates of tis- sues. 7 The study showed that diet supplemented with vitamin E, selenium, trolox C, ascorbic acid palmitate, acetylcysteine, Beta-carotene, canthaxanthin, coen- zyme Q0, coenzyme Q~o, and (+)-catechin provided better protection against heme protein oxidation than diet supplemented with fewer varieties and decreased concentrations of antioxidants.
To approximate more closely the in vivo situation,
fresh blood and tissue slices without incubation can be studied in similar experiments. In this study rats were fed diets supplemented with various combinations of antioxidants for 6 weeks before intraperitoneal injec- tion with CBrCI3, a potent inducer of oxidative dam- age. Protection against oxidative damage by multiple antioxidants was evaluated by measuring the formation of oxidized heme proteins (OHP) in rat blood and tis- sue slices under experimental conditions close to the in vivo state.